Table 1. List of antibodies and stains used for immunohistochemistry.
| Target | Source | Company | Product Number | Dilution | Tissue |
|---|---|---|---|---|---|
| α-smooth muscle actin (αSMA) | Rabbit | AbCam | ab5694 | 1:200 | FM |
| CD31 | Rat | BD Biosciences | 550274 | 1:50 | FM |
| Esm-1 | Goat | R&D Systems | AF1999 | 1:100 | FM |
| F4/80 | Rat | AbCam | ab16911 | 1:200 | IHC-Fr |
| GFAP | Rabbit | AbCam | ab7260 | 1:200 | IHC-Fr |
| Isolectin GS-IB4, Alexa Flour 568 Conjugate | ThermoFisher | I21412 | 1:100 | FM, IHC-P, IHC-Fr | |
| Isolectin GS-IB4, Alexa Flour 488 Conjugate | ThermoFisher | I21411 | 1:50 | Cardiac Perfusion | |
| NG2 | Rabbit | Sigma Aldrich | ZRB5320 | 1:500 | FM |
| Rhodopsin | Mouse | AbCam | ab5417 | 1:50 | IHC-P |
| Secondary Antibodies | Source | Company | Product Number | Dilution | Conjugate |
| Rabbit | Donkey | Jackson Immuno. | 711-295-152 | 1:200 | Rhodamine Red-X |
| Rabbit | Donkey | Jackson Immuno. | 711-605-152 | 1:200 | Alexa-647 |
| Rat | Donkey | Jackson Immuno. | 712-545-150 | 1:200 | Alexa-488 |
| Goat | Donkey | Jackson Immuno. | 705-545-147 | 1:200 | Alexa-488 |
| Mouse | Donkey | Jackson Immuno. | 715-545-150 | 1:200 | Alexa-488 |
Abbreviations: FM: Flatmount; IHC-P: Paraffin sections; IHC-Fr: Cryosections