(A) Western blot quantification of reduced SufT protein level (green bars) and decreased aconitase (Acn) activity (brown bars) was shown in SufT-KD strain, with increasing concentration (ng/mL) of ATc (0, 50, 100, 200 and 400). Level of SufT and enzymatic activity of Acn are normalized to Acn abundance from western blot. B) RT-qPCR based expression level of the suf operon (sufR, sufB, sufD, sufC, sufS, sufU), iscS, and anti-oxidant machinery (katG, sodA and ahpC) in SufT-KD strain. Horizontal dotted line indicates expression level below 1.5-fold considered as basal level. Data are shown as mean ±SD from triplicate reaction. (C) Acn activity was measured from cell free lysates derived from control and SufT-KD strains exposed to ATc (200 ng/mL) for 72 h and subjected to Fe-S reconstituted (Fe2+) and Dipyridyl (DIP) treatment. UT; untreated. D) Acn activity of UT, H2O2 and H2O2+Catalase treated cell lysates from control and SufT-KD strains were measured after 48 h of ATc (200 ng/mL) treatment. Student’s t test (two tailed) was performed here with p values, *p = 0.05,**p = 0.01 and ***p = 0.001.