Fig 6. Trehalose treatment increased metabolites related to defense and wounding in tomato xylem sap.
LC-MS/MS determined concentrations of hormones, oxylipins, and other metabolites in xylem sap collected from cv. Bonny Best tomato plants that were mock-inoculated (H2O), inoculated with R. solanacearum GMI1000 when wilt symptoms first appeared (Rs DI = 1), or had been treated at the roots with 30mM trehalose (Tre (6h), etc.) Differences from water-treated control plants were determined by one-way ANOVA Fisher’s LSD multiple comparison test: *, P = .01-.05; **, P = .001-.01; ***, P = .0001-.001; ****, P < .0001. Data shown represent the means of samples from 4–6 individual plants. Bars indicate mean, vertical lines show standard deviation. A) SA = salicylic acid; B) BA = benzoic acid; C) COUMA = coumaric acid; D) TA = Traumatic acid; E) 12-OPDA = 12-oxo-8(Z),13(Z)-phytodienoic acid; F) JA = jasmonic acid; G) JA-Ile = jasmonic acid isoleucine; H) 9-HOT = 9(S)-hydroxy-10(E),12(Z),15(Z)-octadecatrienoic acid; I) 9-HOD = 9(S)-hydroxy-10(E),12(Z)-octadecadienoic acid; J) 13-HOD = 9(S)-hydroxy-10(E),12(Z)-octadecadienoic acid; and K) 13-KOD = 13-oxo-9(Z),11(E)-octadecadienoic acid.