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. 2022 Apr 27;8(17):eabj5586. doi: 10.1126/sciadv.abj5586

Fig. 3. Inhibition of LH-projecting adBNST neurons induces anxiety-like behavior.

Fig. 3.

(A) Scheme of experiments. (B) Representative images of hM4Di-mCherry expression in the adBNST (left) and AAV2 injection site visualized by coinjection of fluorescent beads in the LH (right). Scale bars, 300 μm (left) and 100 μm (right). AC, anterior commissure; EP, entopeduncular nucleus; lv, lateral ventricle, MEA, medial amygdala; opt, optic tract; Str, striatum. (C) Voltage-clamp recordings of resting membrane potential and membrane resistance in hM4Di-expressing adBNST neurons. Data are expressed as means ± SEM (n = 6 cells from 4 mice). Statistical significance was evaluated using two-tailed paired Student’s t test. *P < 0.05. (D to G) Behavioral analyses using OFT (D), EPM (E), and LDB (F), and emotionality z score across these tests (G). (H) Mechanical thresholds in the von Frey test. Box-whisker plots show the median, interquartile range, and 10th to 90th percentiles. Statistical significance was evaluated using two-tailed unpaired Student’s t test or two-tailed Mann-Whitney test (mCherry: n = 11; hM4Di: n = 10). *P < 0.05; **P < 0.01; ****P < 0.0001. Details of statistical data are provided in table S1.