| Magnification |
×130,000 |
| Voltage (kV) |
300 |
| Microscope |
Titan Krios (Aarhus University) |
| Camera |
Gatan K3 |
| Physical pixel size (Å/pix) |
0.66 |
| Electron exposure (e–/Å2) |
60 |
| Defocus range (μm) |
0.7–1.8 |
| Number of movies |
3,918 |
| Initial particle images (no.) |
470,103 |
| Final particle images (no.) |
104,643 |
| Symmetry imposed |
C1 |
| Map resolution (Å) |
3.1 |
| FSC threshold |
0.143 |
| Map resolution range (Å) |
2.7–4.5 |
|
Refinement
|
|
| Initial model used (PDB code) |
ATP8B1: I-TASSER homology model based on 6ROHCDC50A: 6K7L |
| Model resolution (Å) |
3.3 |
| FSC threshold |
0.5 |
| Map sharpening B factor (Å2) |
–84 |
| Model composition |
|
| Non-hydrogen atoms |
11,868 |
| Protein residues |
1,439 |
| Ligands |
1 MG, 1 BEF, 4 Y01, 4 NAG, 1 BMA |
|
B factors (Å2, min/max/mean) |
|
| Protein |
33.89/136.87/67.09 |
| Ligand |
41.58/110.52/60.89 |
| R.m.s. deviations |
|
| Bond lengths (Å) |
0.002 |
| Bond angles (°) |
0.492 |
| Validation |
|
| MolProbity score |
1.43 |
| Clashscore |
4.74 |
| Poor rotamers (%) |
0.08 |
| Ramachandran plot |
|
| Favored (%) |
96.92 |
| Allowed (%) |
3.08 |
| Disallowed (%) |
0.0 |
