Figure 7.

Impact of calcium, albumin co-administration on LA induced changes in fatty acids, calcium, albumin, DAMPs, coagulation abnormalities, and multisystem organ failure

(A–L) Violin plots of control mice (Con), 48 h after LA administration (LA), and 48 h after administration of LA incubated with albumin and calcium (LA + Ca,Alb). Shown are serum linoleic acid measured by GC-MS (A), total non-esterified fatty acids measured using the colorimetric LabAssay ™ NEFA kit (NEFA; B), unbound fatty acids (C), total calcium (Ca; D), Albumin (Alb.; E), ionized calcium (Ca_i; F), The DAMPs shown are double stranded DNA (ds-DNA; G), and histone-DNA complexes (H). Effects on coagulation parameters including platelets (I) and activated clotting time (ACT; J) are also shown. Carotid pulse distention (Pulse dist. K); a measure of shock, rectal temperature (L); a measure of severe inflammatory response, and blood urea nitrogen (BUN, M) are also shown. P values calculated by a Mann-Whitney U test are shown above corresponding violin plots.