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In vivo (a) THG (green) and (b-f) TPF (red) imaging of ICG in the vessel of a rat's ear. (b) TPF image before the injection of ICG. (c-f) TPF images of intravascular ICG at the (c) 0.5 min, (d) 1.5 min, (e) 10 min, and (f) 15 min post-injection.
