10%, 30-minute extraction silk foams loaded with 1 mg of DNX and water vapor annealed for 4 hours were evaluated. Complement dependent cytotoxicity of DNX release product from (A) hours 0–1, (B) hours 8–24, (C) day 2–3, (D) day 5–7, and (E) to day 28 of release study. Concentration reported are calculated directly from the UV absorbance at 280 nm or enzyme-linked immunosorbent assay and not adjusted for carry over volume to represent the actual DNX concentration within the assay. Data are presented as mean ± standard deviation of three independent samples. Cytotoxicity data are normalized to complement only control and lysed cell control. *p < 0.05 based on a t-test.