Fig. 4.

Analysis of effects of the test compounds on the growth of T. gondii in vitro. Equal amounts of freshly extracted tachyzoites of T. gondii (constitutively expressing Yellow Fluorescent Protein) were inoculated into human foreskin fibroblasts cell line (HFF cells) in culture and compounds at 11 μM final concentration were added immediately after infection. Control infected cells were treated with volumes of DMSO equivalent to those used in the compound-treated cultures. Atovaquone reconstituted in DMSO was added to a separate set of wells as a positive control at 0.5 μM final concentration. The cultures were analyzed by fluorescence microscopy for parasite infectivity and proliferation after 48 h of incubation. Parasite fluorescence quantification was done using Image J software and the mean percent parasite inhibition values of each compound were calculated. The point numerals in the image depict the last two digits of the JS-2- compound series. The data shown represent means of three independent experiments. Bars represent standard errors of the mean.