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. 2022 Apr 16;19:6–20. doi: 10.1016/j.ijpddr.2022.04.001

Fig. 5.

Fig. 5

Effect of varying concentrations of the test compounds on the growth of C. parvum in HCT-8 cells. Equal amounts of freshly excysted sporozoites of C. parvum were inoculated into HCT-8 cells in culture and varying concentrations of (A) JS-2-44, (B) JS-2-30, (C) JS-2-27, (D) JS-2-28 and (E) JS-2-22 were added at the time of infection (green solid line) or added 2 h post- infection (p.i.) (green dashed line). Control infected cells were treated immediately p.i. with volumes of DMSO equivalent to those used in the compound-treated cultures. Varying concentrations of paromomycin (PMO) added at infection (red solid line) or 2 h p.i. (red dashed line) served as the positive control. The cultures were analyzed for parasite infectivity and proliferation by an immunofluorescence assay after 72 h of culture. The fluorescence generated by intracellular C. parvum parasites was quantified and used to calculate the mean percent parasite inhibition values of each compound concentration relative to the DMSO-treated controls. The data shown represent means of three independent experiments. Bars represent standard errors of the mean.(For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)