Extended Data Fig. 4. In silico metabolic modelling of Fn-host tumor cell interaction.
a, Maximum flux changes for exchange reactions of F. nucleatum secreted metabolites. Calculated flux ranges from the FVA results of the F. nucleatum monoculture model were subtracted from FVA results of the HT-29-Fn co-culture model. No minimum flux changes were observed. b, Minimum and maximum flux changes for exchange reactions of HT-29 secreted/uptaken non-essential amino acids and other metabolites. Calculated flux ranges from the FVA results of the HT-29 mono-culture model were subtracted from flux ranges of the HT-29-Fn co-culture model. c, Minimum and maximum flux changes for exchange reactions secreted/uptaken essential amino acids in the HT-29 model. Calculated flux ranges from the FVA results of the HT-29 mono-culture model were subtracted from flux ranges of the HT-29-Fn co-culture model. d, Minimum and maximum flux changes for intracellular reactions of F. nucleatum. Calculated flux ranges from the FVA results of the F. nucleatum mono-culture model were subtracted from FVA results of the HT-29-Fn co-culture model. Cutoff for displayed reactions was -2 and 2 mmol*dGW-1*hr-1. e, Minimum and maximum flux changes for intracellular reactions of HT-29. Calculated flux ranges from the FVA results of the HT-29 mono-culture model were subtracted from flux ranges of the HT-29-Fn co-culture model. Cutoff for displayed reactions was -10 and 10 mmol*dGW-1*hr-1.