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. 2022 Apr 20;604(7907):749–756. doi: 10.1038/s41586-022-04638-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, Results of a CRISPR-based synthetic lethal screen in RPE1-hTERT TP53^−/− Cas9 CCNE1-2A-GFP (C2) cells with CCA and ΔBF scores plotted. b, Dot plot of the synthetic lethal hits from three screens. The size of the dots is proportional to the ΔBF score and blue indicates a hit using CCA (Jenks ranks > 2). c, Volcano plot of gene dependencies in cancer cell lines from the DepMap project grouped according to their CCNE1 amplification status. d, Clonogenic survival assays of the indicated RPE1-hTERT TP53^−/− Cas9 cell lines transduced with lentivirus expressing sgRNA targeting LacZ (LacZ-sg1) or PKMYT1 (PKMYT1-sg4) or doxycycline-inducible sgRNA-resistant Flag alone (−), Flag–PKMYT1 or Flag-PKMYT1^N238A. Top, representative images of plates stained with crystal violet. Bottom, quantification of clonogenic survival assays. Data are mean ± s.d. (n = 3).

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