Extended Data Fig. 3. Expression of vascular niche components in lung metastasis.
a, Overview of the process used to narrow down gene candidates of the pro-metastatic vascular niche in lungs. b, Relative expression of four vascular niche factors in lung ECs, fibroblasts (Fibro), bone marrow-derived cells (BMDC) and epithelial cells (Epi) isolated from lungs harboring metastasis (week 3), as in Fig. 3a. Data are means with s.e.m. from 3 mice (Lama1 and Opg) or 4 mice (Inhbb and Scgb3a1) per group. c, Expression kinetics of niche components during metastatic colonization of lungs. Means with s.e.m. from 3 mice (control) or 4 mice (metastasis week 1, 2 and 3) for each group are shown. d, Relative expression of niche factors in ECs isolated from lungs of healthy control mice or mice harboring metastasis and treated with IgG or anti-VEGF antibody (B20). Data are means with s.e.m. from 3 mice per group. e, Relative expression of the niche factors in lung ECs (n= 4 mice) and the mouse mammary tumor cells 4T1 and E0771 (n= 3). Means with s.e.m. are shown. f, Experimental setup to analyze kinetics of vascular niche activation. g, Expression kinetics of four vascular niche factors in lung ECs in context of spontaneous metastasis from mammary tumors to lungs. Shown are z-scores based on qPCR (top), metastatic burden in mouse lungs at different time points based on ex vivo bioluminescence (middle, n= 3 lungs for day 6, 11, 18, 24; n= 4 lungs for day 32 and n= 6 lungs for day 42 post injection) and primary tumor growth measured by bioluminescence (bottom, n= 5 for day 6, n= 23 for day 11, n= 19 for day 18, and n= 18 for day 24 post injection) or by caliper (mm3, bottom, n= 18 for day 18, and n= 17 for day 24 post injection). Boxes show median with upper and lower quartiles and whiskers indicate maximum and minimum values.