Figure 4.

Overexpression of miR-10/b prevents renal fibrosis

(A) Schematic diagram of the animal experimental procedure.

(B and C) miR-10a (B) and -10b (C) expression by quantitative real-time PCR in kidney from STZ-treated mice. Lentivirus expressing miR-10a or -10b mimic (10a or 10b mimic) was used to overexpress miR-10a or 10b, and lentivirus expressing a nonsense sequence was used as a negative Ctrl (NC mimic) (n = 6).

(D and E) Representative images of H&E staining (D) and Masson’s trichrome and Sirius-red staining (E) in kidney sections from lentivirus-treated DKD mice. Scale bar, 40 μm.

(F) Fibrotic area was calculated in (E). n = 6 in each group.

(G and H) Representative images of transmission electron microscopy in kidney sections from lentivirus-treated DKD mice above (G), and foot-process weight was quantified (H). Scale bar, 1 μm.

Data were expressed as means ± SEM. Student’s t test was used for the comparison of two groups. ∗p < 0.05, ∗∗p < 0.01.