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. 2022 Apr 27;12:6875. doi: 10.1038/s41598-022-10869-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Profiles of all species detected using our method, IDSeq, and 16S sequencing. (A–C) Blot plots show the percentages of reads mapped to top 10 most abundant species using (A) our method, (B) IDSeq, and (C) 16S PCR subcloning and sequencing. Species were inferred from 16S sequence data by 100% sequence matches to the organisms indicated.