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. 2022 Apr 27;13:2276. doi: 10.1038/s41467-022-29964-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Fit of a single subunit PDB reference ribbon structure to the overall models obtained by either method. Note the exposed helices in both models that are not contained within the calculated model density. These data confirm that the matrix-landing sample is no different than the result obtained by conventional negative staining TEM. b Expanded view of a single GroEL subunit where matrix-landed reconstruction is gray and conventionally prepared is yellow. Ribbon structure taken from PDB:5W0S.