Fig. 3. N- and C-terminal cis-cleavage mutants are inhibited differently by GC376 and boceprevir.

a Schematics of the four different constructs; “no mut.” has functional N- and C-terminal cleavage sites leading to complete P protein disruption that can be recovered by protease inhibitor treatment; N,C-term has mutated N- and C-terminal cleavage sites and constitutive activity regardless of inhibitor treatment; N-term and C-term constructs have glutamine (Q) to asparagine (N) substitutions in the N- and C-terminal cleavage sites, respectively, which can be recovered differentially by protease inhibition. b, c GC376 and boceprevir dose-response experiments, respectively, with the constructs described in a (n = 3 biologically independent replicates per condition with individual data points shown and average values represented by histogram bars).