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. 2022 Apr 27;5:391. doi: 10.1038/s42003-022-03277-0

Table 2.

cloning primer for lentivirus plasmids.

Name Sequence (5'-3' direction)
LV-L-for CTGTTTTGACCTCCATAGAAGATTCTAGAGCTAGCATGGAAGTCCACGATTTTGAG
L-LV-rev GAGGGAGAGGGGCGGATCCCCTTAATTAATTAATCTCTCCAAGAGTTTTCCTC
LV-GFP-for GACCTCCATAGAAGATTCTAGAGCTAGCATGAGCAAGGGCGAGGAACTG
L-33bp-after-HpaI-rev GATGTTGGGATGGGATTGGC
LV-P-for CTGTTTTGACCTCCATAGAAGATTCTAGAGCTAGCATGGATAATCTCACAAAAGTTC
P-LV-rev GAGGGAGAGGGGCGGATCCCCTTAATTAACTACAGAGAATATTTGACTCTCGC
GGSG-BatCoV-On-for GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGAAGCGTCGCCAGTGCTGCGC
BatCoV-GGSG-On-rev GCCGGATCCACCGCCTGAGCCGCCTCCGGACCCTCCTCGAAACATAGATTGAAATTTACCTTG
GGSG-HCoV-229E-On-for GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGAGTATCTTATGGCTCAACGCTCCAA
HCoV-229E-GGSG-On-rev GCCGGATCCACCGCCTGAGCCGCCTCCGGACCCTCCAAACATGGATGTAGTCTTACCAGATTG
GGSG-HCoV-NL63-On-for GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGAATCAGTTACAATAGTACCTTGCAAAGC
HCoV-NL63-GGSG-On-rev GCCGGATCCACCGCCTGAGCCGCCTCCGGACCCTCCAAGCCCGAATATAACCTTTCCTG
GGSG-MERS-On-for GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGATCAATCACTAGCGGTGTATTGC
MERS-GGSG-On-rev GCCGGATCCACCGCCTGAGCCGCCTCCGGACCCTCCGTATGTTACTTTTCTTACACCGGAC
GGSG-MHV-A59-On-for GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGATCAGTCACCACTTCATTTCTCC
MHV-A59-GGSG-On-rev GCCGGATCCACCGCCTGAGCCGCCTCCGGACCCTCCTTTTATTACTCTAGTCCTTTTACTCTGCAG
GGSG-Polio-On-for GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGAACCATTCGGACAGCAAAGGTAC
Polio-GGSG-On-rev GCCGGATCCACCGCCTGAGCCGCCTCCGGACCCTCCAGGTCTCATCCACTGGATTTC
GGSG-SARS1-On-for GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGAAGTATCACGTCTGCTGTGC
SARS1-GGSG-On-rev GCCGGATCCACCGCCTGAGCCGCCTCCGGACCCTCCTTTGACTATTTTTTTGAACTTACCTTG
BatCoV-On-N-term-QtoN-for GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGAAGCGTCGCCAGTGCTGCGCTCAACGCGGGTCTTACTC
HCoV-229E-On-N-term-QtoN-for GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGAGTATCTTATGGCTCAACGCTCAACGCCGGCTTGCGC
HCoV-NL63-On-N-term-QtoN-for GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGAATCAGTTACAATAGTACCTTGAACAGCGGACTG
MERS-On-N-term-QtoN-for GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGATCAATCACTAGCGGTGTATTGAACAGTGGTTTGGTC
MHV-On-N-term-QtoN-for GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGATCAGTCACCACTTCATTTCTCAACTCCGGGATAG
Polio-On-N-term-QtoN-for GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGAACCATTCGGACAGCAAAGGTAAACGGACCAGGGTTC
SARS1-On-N-term-QtoN-for GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGAAGTATCACGTCTGCTGTGCTCAACTCAGGCTTCAG