Figure 3.

XF analyses of BMDMs and HMDMs yield insight into metabolic profiles of macrophages after LPS ± IFNγ and IL-4-activation

(A and B) Normalized (to relative Hoechst⁺ objects) ECAR, with injections of glucose, oligomycin, FCCP, and antimycin A/rotenone/Hoechst for BMDMs (A) and HMDMs (B).

(C and D) Normalized (to relative Hoechst⁺ objects) OCR with same injections as for ECAR for BMDMs (C) and HMDMs (D).

(E and G) Metabolic profiles outlining basal respiration and glycolysis for BMDMs (E) and HMDMs (G).

(F and H) Mitochondrial and glycolytic contribution to overall ATP production in BMDMs (F) and HMDMs (H); n = 6 mice or n = 6 donors were included with four to five technical replicates each. Values shown as mean ± SEM calculated from the average of technical replicates per mouse/donor. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 by one-way ANOVA with Dunnett’s post hoc test for multiple comparisons. For (F) and (H), significance on top of bar graphs indicates changes in total ATP production rate; significance within bars indicates significant differences between either the glycolytic or mitochondrial contribution to ATP production rate compared with N.