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. 2022 Mar 28;2(4):100192. doi: 10.1016/j.crmeth.2022.100192

Figure 5.

Figure 5

Uptake of fluorescent probes provides additional insight into macrophage metabolism

(A and B) Representative images of BMDM (A) and HMDM (B) staining by fluorescent dyes and uptake of fluorescent nutrient analogs as assessed by multi-mode reader. Scale bar represents 200 μm.

(C–F) Fluorescence intensity of 2NB-DG (C and E) and BODIPY C16 (D and F) uptake by BMDMs (C and D) and HMDMs (E and F) as examined by flow cytometry, correlated with relevant parameters of XF analysis.

(G–J) Fluorescence intensity of MitoTracker Green (G and I) and TMRM (H and J) analysis as examined by flow cytometry and correlations with relevant parameters of XF analysis in BMDMs (G and H) and HMDMs (I and J).

Data are shown as mean ± SEM For graphs of fluorescent probes, each dot marks a separate mouse (n = 9) or donor (n = 8). ΔMFI was calculated as MFI (median fluorescence intensity) of sample – MFI of unstained control. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 by one-way ANOVA with Dunnett’s post hoc test for multiple comparisons. Correlations were fitted using a simple linear regression model.