Skip to main content
. 2022 Apr 25;2(4):100199. doi: 10.1016/j.crmeth.2022.100199

Figure 3.

Figure 3

Electron microscopy of synaptic vesicles labeled with C2-XL-HRP

(A and B) Representative electron micrographs of terminals loaded with 4 μM C2-XL-HRP by high K+ stimulation followed with (B) or without (A) a 1-min EGTA wash step. Post-stimulation recovery time was 10–20 min. Arrows indicate endosome-like vacuoles.

(C) Distributions of vesicle diameters in terminals loaded with C2-XL-HRP by high K+ stimulation. Black bars, C2-positive vesicles (n = 359); white bars, C2-negative vesicles (n = 783).

(D) Distributions of vesicle diameters in terminals loaded by spontaneous activity (overnight incubation with C2-XL-HRP). Black bars, C2-positive vesicles (n = 199); white bars, C2-negative vesicles (n = 492).