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. 2022 Apr 25;2(4):100199. doi: 10.1016/j.crmeth.2022.100199

Figure 6.

Figure 6

TIRFM of synaptic vesicles labeled with C2-TR

(A) Single frame (left) and average of 600 frames (4 recordings, right). Boxed region shows expanded views in (C).

(B) Histogram of spot intensities. Intensities (129 spots in 3 cells) were normalized to mean intensity of each cell and then pooled.

(C) Expanded region from (A). Red circles indicate spots that appear or disappear during one recording. Green arrows point to sites of exocytosis. Images were low-pass filtered.

(D) Image sequence of a single fusion event. Time is relative to the moment of fusion. Dotted concentric circles in the rightmost panel depict center and annulus regions.

(E) Fluorescence changes in center (filled) and annulus (open) of the fusion event in (D). Arrow indicates moment of fusion.

(F–I) Fusion events versus undocking events. (F) Image sequence of averaged fusion events (n = 10 events in 3 cells). (G) Image sequence of averaged undocking events (n = 10 events in 3 cells). (H) Center-minus-annulus trace for fusion events in (F). (I) Center-minus-annulus trace for undocking events in (G). Error bars, SEM.