Vital imaging of nasal mucosa after application of gGlu-HMRG demonstrates labeling of OE
(A) gGlu-HMRG is hydrolyzed to HMRG by the catalytic activity of GGTs.
(B) Fluorescent imaging of nasal whole mounts loaded with 10 μM Glu-HMRG captured before and 5, 15, and 30 min after probe application reveal signal in areas comparable to OE.
(C) Relative fluorescence intensity of nasal mucosa of OE vs. RE measured every 5 min after application of gGlu-HMRG. The data are shown as the mean ± SEM (n = 5). Statistical differences were calculated in initial time vs 30 min and initial time vs 60 min ∗p < 0.05. D = dorsal, V = ventral, A = anterior, p = posterior, OB = olfactory bulb, scale bars = 2 mm.