REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
PGP 9.5/UCHL1	Proteintech	Cat# 14730-1-AP; RRID:AB_2210497
TUJ1	BioLegend	Cat# 801201; RRID: AB_2313773
Tubulin beta Ⅳ (TUBB4)	Abcam	Cat# ab179509; RRID:AB_2716759
Tubulin beta Ⅳ (TUBB4)	Sigma-Aldrich	Cat# T7941; RRID: AB_261775
CK19	DSHB	Cat# Troma-Ⅲ-c; RRID: AB_2133570
CYP2A6	Invitrogen	Cat# MA5-25758; RRID: AB_2723032
GGT7	Proteintech	Cat# 24674-1-AP; RRID: AB_2879667
Donkey anti-Mouse IgG-Alexa Fluor 488	Invitrogen	Cat# A-21202 RRID: AB_141607
Donkey anti-Rabbit IgG-Alexa Cy3	Jackson ImmunoResearch Laboratories	Cat# 711-165-152; RRID: AB_2307443
Donkey anti-Rat IgG-Alexa Cy5	Jackson ImmunoResearch Laboratories	Cat# 712-175-153: RRID: AB_2340672
Chemicals, peptides, and recombinant proteins
Coumarin	Sigma-Aldrich	Cat# C4261 Cas 91-64-5
ProteoGREEN™-gGlu	GORYO Chemical, Inc	Cat# GC801
Hematoxylin Solution	Sigma-Aldrich	Cat# 105175
Eosion Solution	Sigma-Aldrich	Cat# 2853
8-Methoxypsoralen	Sigma-Aldrich	Cat# M3501 Cas 298-81-7
GGsTop	Tocris Bioscience	Cat# 4452
Dichlobenil	Sigma-Aldrich	Cat# D57558 Cas 1194-65-6
Methimazole	Sigma-Aldrich	Cat# M8506, Cas 60-56-0
Critical commercial assays
Quick-RNA Miniprep	ZYMO RESEARCH	Cat# R1054
GoScript Reverse Transcriptase	Promega	Cat# A5003
2X SYBR Green qPCR Master Mix	Genesee Scientific	Cat# 42-116PG
Deposited data
Single-cell RNA seq	Schwob Lab	GSE166612
Single-cell RNA seq	Durante et al.	GSE139522
Experimental models: Organisms/strains
Mouse: Crl:CD1(ICR)	Charles river	Strain #022 RRID:IMSR_CRL:022
Mouse: B6;129P2-Omptm3Mom/MomJ (OMP-GFP)	Jackson Laboratory	Strain #006667 RRID:IMSR_JAX:006667
OMP-tTA;TetO-DTA transgenic mouse	Child, et al. https://www.jneurosci.org/content/38/31/6806.long	N/A
Oligonucleotides
Primer for Cyp2a5 forward: 5′-TCG GAAGACGAACGGTGCTTTT-3′	This paper	N/A
Primer for Cyp2a5 reverse: 5′-GCTT CCCAGCATCATTCGAAGC-3′	This paper	N/A
Primer for Ggt1 forward: 5′-CTG GAAGAGCCATGAAGAATCG-3′	This paper	N/A
Primer for Ggt1 reverse: 5′-CACG GAACCACCTTCCTGTA-3′	This paper	N/A
Primer for Ggt7 forward: 5′-GTA GTCCGACCAGCAGAAGG-3′	This paper	N/A
Primer for Ggt7 reverse: 5′-GTG AACTCACTGTCAACCTGC-3′	This paper	N/A
Primer for Gapdh forward: 5′-TGT GTCCGTCGTGGATCTGA-3′	This paper	N/A
Primer for Gapdh reverse: 5′-TTGC TGTTGAAGTCGCAGGAG-3′	This paper	N/A
Software and algorithms
GraphPad Prism 8	Graphpad	https://www.graphpad.com/scientificsoftware/prism/RRID:SCR_002798
ImageJ	U.S. National Institutes of Health	https://imagej.nih.gov/ij/RRID:SCR_003070
SPOT ver 5.2	SPOT imaging	https://www.spotimaging.com/RRID:SCR_014313
Adobe photoshop	Adobe	RRID:SCR_014199
Adobe illustrator	Adobe	RRID:SCR_010279
Zen 3.0	Zeiss	RRID:SCR_013672
Other
NanoDrop	Thermofisher Scientific	RRID:SCR_015804
Confocal Microscope LSM800	Zeiss	SCR_015963
Nikon Microphot-SA	Nikon	https://www.microscope.healthcare.nikon.com/products/upright-microscopes/eclipse-ni-u
CFX 96 system	Bio-Rad Laboratories	Cat# 1855196J1 RRID:SCR_018064