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. 2022 Apr 7;25(5):104218. doi: 10.1016/j.isci.2022.104218

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© 2022 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Mapping the main output regions of CR neurons in the PIL

(A) A series of representative images showing CR projections from a CR-cre mouse injected with AAV2/9-CAG-FLEX-ArchT-GFP into the PIL, illustrating the principal projections of the PIL CR neurons (left image). Scale bar: 1.0 mm.

B and C) Enlarged view of Arch-GFP soma within PIL and GFP⁺ axon fibers in ZI, LA, Au, and CeA (B) and corresponding c-Fos expression after kindling-induced GS (C). Scale Bar: 200 μm. PIL, posterior intralaminar thalamic nucleus; Au, auditory cortex; LA, lateral amygdala; CeA, central amygdaloid nucleus; ZI, zona incerta.