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. 2022 Apr 27;12:6902. doi: 10.1038/s41598-022-10914-5

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The HML-6 envelope transcript is overexpressed in GBM cell lines. (A) ERVK3-1 is located in the envelope region of the HML-6 provirus and encodes a small peptide (~ 140–190 amino acids). (B) Two sets of primers for the HML-6 provirus localize to the ERVK3-1 envelope region. (C) Quantitative Polymerase Chain Reaction (PCR) demonstrates significant overexpression of HML-6 in the adherent A172 glioblastoma cell line and patient-derived glioma neurosphere cultures (GBM 28 and GBM43). Data represents mean + SEM of triplicates and analyzed by multiple ANOVA. *p < 0.05.