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. 2022 Apr 27;12:6902. doi: 10.1038/s41598-022-10914-5

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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HML-6 is overexpressed in several glioma cell lines compared to astrocytes (A). RNA-in situ hybridization confirms presence of HML-6 provirus RNA in the nucleus (DAPI) and cytoplasm of glioblastoma cells. The adherent A172 glioblastoma cell lines (B) and neurosphere patient-derived glioblastoma cell lines, GBM28 (C) and GBM43 (D) have increased levels of HML-6 transcripts compared to astrocytes.