Fig. 2.

Observer-independent identification of cytoarchitectonic borders. a Postmortem brain number 4 (Table 1). The sectioning plane is marked in red. b Coronal section stained for cell bodies with ROI (black rectangle). This ROI was digitized and subsequently transformed into a GLI image. c Inverted GLI image. d Equidistant traverses were calculated and GLI profiles were extracted along these traverses. e Mahalanobis distances as a function on the position of the profile along the cortical ribbon (example for a block size of 20 profiles); significant maximum at profile 55. f Projection of the identified border between areas Ph1 and Ph3 to the original image