Fig. 5.

Effects of maternal diet on neuroprotective responses following tMCAO. (a) Volume of brain injury 72 h after tMCAO reperfusion in pups born to mothers fed a standard (grey), n-3 (blue) or n-6 (yellow) enriched diet. (b) Representative examples of anterior-to-posterior injury distribution in neonatal brain following tMCAO (Nissl staining). (c) Spectrin cleavage 24 h after reperfusion. Western Blot shows representative examples of spectrin cleavage via caspase-3-dependent (125KDa band, left) and calpain-dependent (150KDa band, right) in injured (ipsi) and non-injured (contra) brain cortex. Data presented as mean ± SD. Kruskal–Wallis test followed by Dunn’s post-hoc test (a) or two-way ANOVA followed by Tukey’s post hoc test (c) was performed to compare groups. Individual p-values are listed within figures for data that are significantly different. N = 7–15/diet