Table 2.
Primer sequences for qRT-PCR.
| Genes | Forward primer sequence | Reverse primer sequence |
|---|---|---|
| homo-BMP4 | AGCTTCCACCACGAAGAACAT | AAGCCCCTTTCCCAATCAGG |
| homo-GPX4 | TTCCCGTGTAACCAGTTCGG | GCCCTTGGGTTGGATCTTCA |
| homo-GAPDH | AGGTCGGTGTGAACGGATTTG | TGTAGACCATGTAGTTGAGGTCA |
| homo-IL-8 | TTTTGCCAAGGAGTGCTAAAGA | AACCCTCTGCACCCAGTTTTC |
| homo-TNFα | ACATACTGACCCACGGCTC | CCTGCCACGATCAGGAAGG |
| homo-IL-13 | CCTCATGGCGCTTTTGTTGAC | TCTGGTTCTGGGTGATGTTGA |
| homo-IL-6 | ACTCACCTCTTCAGAACGAATTG | CCATCTTTGGAAGGTTCAGGTTG |
| homo-IL-4 | CCAACTGCTTCCCCCTCTG | TCTGTTACGGTCAACTCGGTG |
| homo-IL10 | CTCAGCACTGCTCTGTTGC | ACAAGTTGTCCAGCTGATCC |
| mus-ACTIN | GGCACCACACCTTCTACAATGA | ACGCTCGGTCAGGATCTTCA |
| mus-BMP4 | AGCCAACACTGTGAGGAGTT | ATACGGTGGAAGCCCTGTTC |
| mus-GPX4 | ACGAATTCTCAGCCAAGGACA | ACGAATTCTCAGCCAAGGACA |
qRT-PCR real-time reverse-transcriptase polymerase chain reaction, BMP4 bone morphogenetic protein 4, GPX4 glutathione peroxidase 4; IL interleukin; TNF tumor necrosis factor.