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. 2022 Apr 27;12:48. doi: 10.1186/s13568-022-01389-8

Table 2.

Hydroxylation of monoterpene derivatives using recombinant cells of E. coli harboring CYP153A6

Entry Substrate Product Recovered yield (%)
1

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1d

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2d

72
2

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1e

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2e

68
3

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1f

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2f

64
4

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1g

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2e

65
5

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1h

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2f

69
6

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1i

No reaction
7

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1j

No reaction
8

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1k

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2k

39
9

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1l

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2l

65
10

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1m

No reaction
11

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1n

No reaction

Biotransformation conditions: substrates (10.0 mM) were added to the suspension of whole cells of recombinant E. coli (50 mg mL−1) in phosphate buffer (100 mM, pH 8.0) at 28° C

Products were recovered after 5 h of biotransformation