Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 14;12:873020. doi: 10.3389/fonc.2022.873020

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Tan, Xia, Zhang, Xu, Liu, Guo and Liu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

RBM15B-mediated stabilization of TRAM2 mRNA relies on RBM15B methyltransferase activity and m6A modifications in the 3’-UTR of TRAM2 mRNA. (A) LC/MS results showing the m6A methylation level. (B) Dot blot showing changes in m6A methylation. Methylene blue staining was used as a control. (C) The SRAMP database predicted the distribution of m6A sites in TRAM2 mRNA. (D) Plasmids with either WT or MUT m6A sites were constructed. The plasmid was transfected into HEK293T cells. Firefly luciferase activity was measured and normalized to Renilla luciferase activity. (E) m6A-RIP and qRT-PCR analysis of m6A levels in TRAM2 mRNA in HCC cell lines. (F) Changes in TRAM2 mRNA stability after ACT-d treatment of Huh-7 cells. *p < 0.05; **p < 0.01; ***p < 0.001. ns means no significant difference.