Skip to main content
. 2022 Apr 12;17:100311. doi: 10.1016/j.lrr.2022.100311

Fig 1.

Fig 1

Clonal evolution in a patient with CSF3R-T618I CNL to AML with t(4;12)(q12;p13). (A) Bone marrow aspiration smear showing maturing granulopoiesis without an increase in blasts at diagnosis (Wright-Giemsa stain, 1,000x original magnification). (B) Bone marrow aspiration smear showing blasts with small- to medium-size, round nuclei and a blocky chromatin pattern in agranular cytoplasm at AML transformation (left, Wright-Giemsa stain, 1,000x original magnification; right, peroxidase stain, x1,000 original magnification). (C) FISH analysis was performed using break-apart probes for the 4q12 region on cultured peripheral blood cells. The tri-color break-apart probe (5′FIP1L1 in green, 3′FIP1L1→5′PDGFRA in orange, and 3′PDGFRA in aqua) showed 1 triple fusion, 1 green-orange fusion (arrow), and 1 separate aqua signal (arrow), which confirmed CHIC2 or PDGFRA gene rearrangement (presumably translocation to derivative chromosome 12) in 58% of the nuclei.