FIG. 2.

Replacement of the fxbA gene in M. smegmatis mc²155. (A) Strategy used to replace the fxbA gene by double crossover. The horizontal arrows indicate annealing sites of primers used to confirm the replacement (B1-K1, B9-K2) by using the chromosomal DNA of mutant strain B1 as the template and to generate control fragments by using plamid pGS31 as the template. The expected sizes of the fragments are indicated between the arrows. (B) Confirmation of replacement of the fxbA gene by PCR analysis of DNA from the B3 ΔfxbA mutant.