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. 2022 Feb 21;13(6):1649–1672. doi: 10.1016/j.jcmgh.2022.02.009

Figure 11.

Figure 11

Ferroptosis was enhanced by LPS/D-galactosamine (D-GalN)-induced ALF in Nrf2-/-mice compared with WT mice. (A) Serum ALT and AST levels of WT and Nrf2-/- mice treated with vehicle or LPS/D-GalN (n = 6). (B) H&E staining and 4-HNE immunohistochemistry staining of WT mice and Nrf2-/- mice with or without LPS/D-GalN co-injection (n = 6). (C and D) MDA and SOD assay of WT mice and Nrf2-/- mice with or without LPS/D-GalN co-injection (n = 6). (E) Representative pictures for DMT1 (green) and Ptgs2 (red) immunofluorescence staining from WT and Nrf2-/- mice treated with or without LPS/D-GalN and (F) quantitative results (n = 4–5). (G) Representative Western blots of XCT, GPX4, FSP1, and DHODH from WT and Nrf2-/- mice treated with or without LPS/D-GalN and (H) ratios of each protein to GAPDH. (I) Representative Western blots of CHAC1 and POR from WT and Nrf2-/- mice treated with or without LPS/D-GalN and (J) ratios of each protein to GAPDH (n = 3). All data are presented as means ± SEM. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.Scale bar: 100 μm. DAPI, 4′,6-diamidino-2-phenylindole; D-GalN, D-galactosamine; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.