Isolation of macrophages from mouse skin wounds for single-cell RNA sequencing

. 2022 Apr 19;3(2):101337. doi: 10.1016/j.xpro.2022.101337

SMART Buffer

Reagent	Final concentration	Amount
5× First Strand Buffer of Superscript II	N/A	2 μL
Betaine (5 M)	1.9 M	2 μL
DTT (100 mM)	9.6 mM	0.5 μL
MgCl₂ (1 M)	0.012 M	0.06 μL
Nuclease-free water	N/A	0.64 μL
Total	N/A	5.2 μL