Isolation of macrophages from mouse skin wounds for single-cell RNA sequencing

. 2022 Apr 19;3(2):101337. doi: 10.1016/j.xpro.2022.101337

Antibody Mix

Antigen	Fluorophore	Clone	Stock concentration	Final concentration
CD45	FITC	30-F11	0.2 mg/mL	1.0 μg/mL
CD11b	APC-eFluor780	M1/70	0.2 mg/mL	0.5 μg/mL
F4/80	PE	Cl:A3-1	100 tests	10 μL/test
Trem2	APC	237920	100 tests	10 μL/test
CD301b	PerCP/Cy5.5	URA-1	0.2 mg/mL	4 μg/mL
MHC II	eFluor450	M5/114.15.2	0.2 mg/mL	1 μg/mL