Table 1.
Primary and secondary antibodies used for immunofluorescence staining
| Host | Dilution | Source; catalog number | RRID | |
|---|---|---|---|---|
| Primary antibody | ||||
| HuC/D | Mouse | 1 μg/ml | Thermo Fisher Scientific; A-21271 | AB_221448 |
| MCH | Rabbit | 1:3000 | Phoenix Pharmaceuticals; H-070-47 | AB_10013632 |
| Orexin-A | Goat | 1:250 | Santa Cruz Biotechnology; sc-8070 | AB_653610 |
| GAD67 | Mouse | 1:500 | Millipore; MAB5406 | AB_2278725 |
| VGLUT2 | Guinea pig | 1:500 | Millipore; AB2251-I | AB_2665454 |
| Nesfatin-1 | Sheep | 2 μg/ml | R&D Systems; AF6895 | AB_10972964 |
| CART | Chicken | 1:500 | Millipore; AB5340P | AB_91795 |
| Rax | Guinea pig | 1:2000 | TaKaRa; M229 | AB_2783559 |
| Pax6 | Mouse | 1:100 | DSHB; PAX6 | AB_528427 |
| Nkx2.1 | Rabbit | 1:200 | Santa Cruz Biotechnology; sc-13040 | AB_793532 |
| Nkx2.1 | Mouse | 1:500 | MBL; K0121-3 | AB_592930 |
| Sox1 | Goat | 1:500 | R&D Systems; AF3369 | AB_2239879 |
| Foxg1 | Rabbit | 1:1000 | TaKaRa; M227 | AB_2827749 |
| Nkx2.2 | Mouse | 1:50 | DSHB; 74.5A5 | AB_531794 |
| NK3R | Rabbit | 1:1000 | Novus Biologicals; NB300-102SS | AB_2287128 |
| GFP | Chicken | 1:500 | Thermo Fisher Scientific; A10262 | AB_2534023 |
| Secondary antibody | ||||
| Anti-mouse, Alexa Fluor 488 | Goat | 1:1000 | Thermo Fisher Scientific; A-11029 | AB_2534088 |
| Anti-rabbit, Alexa Fluor 488 | Donkey | 1:1000 | Thermo Fisher Scientific; A-21206 | AB_2535792 |
| Anti-chicken, Alexa Fluor 488 | Donkey | 1:500 | Jackson ImmunoResearch; 703-545-155 | AB_2340375 |
| Anti-goat, Alexa Fluor 488 | Donkey | 1:1000 | Thermo Fisher Scientific; A-11055 | AB_2534102 |
| Anti-mouse, Alexa Fluor 555 | Donkey | 1:1000 | Thermo Fisher Scientific; A-31570 | AB_2536180 |
| Anti-rabbit, Alexa Fluor 555 | Donkey | 1:1000 | Thermo Fisher Scientific; A-31572 | AB_162543 |
| Anti-goat, Alexa Fluor 555 | Donkey | 1:1000 | Thermo Fisher Scientific; A-21432 | AB_2535853 |
| Anti-sheep, Alexa Fluor 555 | Donkey | 1:1000 | Thermo Fisher Scientific; A-21436 | AB_2535857 |
| Anti-mouse, Alexa Fluor 594 | Donkey | 1:1000 | Thermo Fisher Scientific; A-21203 | AB_141633 |
| Anti-rabbit, Alexa Fluor 594 | Donkey | 1:1000 | Thermo Fisher Scientific; A-21207 | AB_141637 |
| Anti-guinea pig, Cy3 | Donkey | 1:500 | Jackson ImmunoResearch; 706-165-148 | AB_2340460 |
| Anti-mouse, Alexa Fluor 647 | Donkey | 1:1000 | Thermo Fisher Scientific; A-31571 | AB_162542 |
| Anti-rabbit, Alexa Fluor 647 | Donkey | 1:1000 | Thermo Fisher Scientific; A-31573 | AB_2536183 |