TABLE 1.

Verification of AF2‐generated models by comparison with experimental structures

Bitopic proteins in Membranome 3.0			Superposition of AF2 models with PDB structures a
Species	N prot b	N AF2 c	N AF2 c	Rover‐AF2 d	Rover‐AF2 e (%)	RMSD f (Å)	Identity g (%)
H. sapiens	2,383	2,368	788	271 ± 184	53 ± 22	1.5 ± 0.9	97 ± 10
A. thaliana	2,105	2069	52	215 ± 106	51 ± 24	1.0 ± 1.0	98 ± 4
S. Cerevisiae	383	374	69	104 ± 21	26 ± 17	1.1 ± 0.5	100 ± 1
D. discoideum	605	598	0	0	0	0	0
E. coli	205	204	34	476 ± 358	83 ± 10	2.8 ± 1.8	99 ± 1
M. jannaschii	77	77	4	131 ± 29	77 ± 9	0.6 ± 0.5	99 ± 1

^a Average values with SD. One PDB entry with the largest number of overlapped residues was selected for each protein.

^b N _prot, number of bitopic proteins.

^c N _AF2, number of AF2 models included in Membranome database.

^d R _over‐AF2, number of overlapping residues between AF2 model and PDB structure.

^e Percentage of overlapping residues between AF2 model and PDB structure.

^f RMSD for Cα‐atoms.

^g Sequence identity between overlapping residues in AF2 models and PDB structures.