Fig. 10. Quantification of ROS production by using fluorescence dye. Fluorescence intensity at 520 nm of E. coli ATCC 25922 cells treated with ZO, 2NZO, and 5NZO, respectively, was measured. Bacteria cells treated with NPs in PBS are used as a blank for fluorescence detection. Three-independent experiments were performed. The data was processed using MARS Data Analysis software (ver. 3.02 R2; BMG Labtech) and relative ROS production by NPs were shown (p < 0.05).
