ERANES and TRANES analysis of the ground state heterogeneity of various two-component systems obtained using C466, C102, C307, C153, tryptophan and tyrosine.

S. no.	Two-component system (A and B)	Comparison of absorption and fluorescence spectral data between individual components		Quantum yield ratio	Fluorescence lifetime ratio	Analysis of ground state heterogeneity using
						ERANES	TRANES
		Difference in absorption maximum (nm)	Difference in fluorescence maximum (nm)
1	C307 and C466	10	31	1.48	4.52	Ground state heterogeneity resolved with an isoemissive point at 483 nm (Fig. S5)	Ground state heterogeneity resolved with an isoemissive point at 483 nm (Fig. S6)
2	C102 and C153	33	61	1.35	1.16	Ground state heterogeneity resolved with an isoemissive point at 508 nm (Fig. S7)	Ground state heterogeneity not resolved due to (i) model-free fitting and (ii) difference in the fluorescence lifetimes of the individual fluorophores, which was comparable to the time resolution (540 ps) of the TCSPC (Fig. S8, 10 and 12)
3	C307 and C153	34	40	0.75	1.29	Ground state heterogeneity resolved with an isoemissive point at 514 nm (Fig. S9)
4	C307 and C102	1	21	1.8	0.55	Ground state heterogeneity resolved with an isoemissive point at 486 nm (Fig. S11)
5	C102 and C466	11	10	2.66	4	Ground state heterogeneity resolved with an isoemissive point at 467 nm (Fig. S13)	Ground state heterogeneity resolved with an isoemissive point at 490 nm (Fig. S14)
6	Tryptophan and tyrosine	5	50	0.7	1.16	Ground state heterogeneity resolved with an isoemissive point at 328 nm (Fig. S15)	—