Fig. 12.

Genetically encoded sensors for neurotransmitters. (a)–(b) Design scaffolds for sensors based on cognate GPCR (a) or E. coli PBP (b). (c) 2P imaging of dopamine dynamics in the mouse cortex using dLight1.2 when mouse performs a visual-motor learning task. Heat map of dLight1.2 expression pattern in layer 2/3 of M1 cortex is overlaid with computationally defined regions of interest colored by the type of responses observed during the task. (d) Average task-induced dLight1.2 transients (bottom) and mouse running velocity (top) aligned to trial onset (0 s). For details see Ref. 210.