Fig. 19.

1P wearable microscopes for cellular-resolution imaging in freely behaving animals. (a) Example 1P miniscope for high-speed, cellular-resolution imaging in freely behaving mice. This implementation is wire-free and weighs 4-5 grams, including a 1.1-gram single-cell lithium-polymer battery. It allows recordings of $\ge 30\min$ duration at 20 Hz, 320 x 320 pixels resolution, x2 pixel subsampling across a $550\mu \mathrm{m}\times 550\mu \mathrm{m}$ FOV. Data is logged onto a MicroSD card for offline analysis. (b) Example raw images of GCaMP6f ${\mathrm{Ca}}^{2+}$ indicator-expressing (top) and computationally identified cells (bottom) in the dorsal hippocampus of control and epileptic mice. Imaging was performed using a tissue-implanted GRIN lens. (c) Spatially tuned activity pattern across 20 example cells from control and epileptic mice trained to run on a 2-m linear track for water reward. Adapted from Ref. 374 with permission from Nature Publishing Group.