Fig. 22.

Large field-of-view, multiregion 2P imaging. (a) FOV available in conventional 2P systems are often confined to $\sim 0.5\mathrm{mm}$, which is smaller than many cortical areas. Larger FOVs (2-mm diameter, or the 5 x 5 mm region of the Diesel2p system⁴¹⁰ provide optical access to multiple cortical areas simultaneously. (b) Neuronal activity can be monitored across these FOVs through rapid beam steering and/or multiplexed beams. (c)–(d) The Diesel2p is a system that provides a large FOV (c) and dual scan engines coupled into a single objective (d) for independent multiplexed imaging and/or 2P optogenetic manipulations of neuronal activity. (e) With large FOV, 2P imaging systems, subcellular resolution is available across a large imaging volume, containing on the order of a million neurons. Adapted from Ref. 410.