TABLE 2.
Killing activities of histatin derivitives against C. albicans
| Peptide | Sequenceb | LD50c (μg/ml) | LD90c (μg/ml) |
|---|---|---|---|
| Histatin 5 | DSHAKRHHGYKRKFHEKHHSHRGY | 7.3 ± 0.52 | 8.3 ± 0.3 |
| P-123 | DSHAKRHHGYKRKF | >80 | >80 |
| P-103 | KRHHGYKRKFHEKHHSHR | 10.7 ± 5.49 | 24.8 ± 8.1 |
| Histatin 9 | RKFHEKHHSHRGYR | 39 ± 15.8 | >80 |
| P-112 | DSHAKRHHGYKR | >80 | >80 |
| P-113unama | AKRHHGYKRKFH | 3.9 ± 0.63 | 7.8 ± 1.33 |
| P-113 | AKRHHGYKRKFH–NH2 | 2.3 ± 0.65 | 4.7 ± 0.16 |
| P-113D | AKRHHGYKRKFH–NH2 | 2.2 ± 0.3 | 4.8 ± 0.13 |
| P-114 | HHGYKRKFHEKH | >80 | >80 |
| P-115 | YKRKFHEKHHSH | >80 | >80 |
| P-116 | KFHEKHHSHRGY | >80 | >80 |
| P-117 | KRHHGYKRKFH | 13.7 ± 6.6 | >80 |
| P-118 | AKRHHGYKRKF | 10.0 ± 6.1 | >80 |
| P-119 | AKRHHGYKRK | >80 | >80 |
| P-120 | AKRHHGYKR | >80 | >80 |
a
P-113unam, P-113 in the unamidated form.
b
The designation–NH2 signifies carboxyl-terminal amidation; all peptides except P-113 and P-113D are not amidated.
c
LD50s, LD90s, and ranges (variances) were calculated as averages of two independent experiments, each of which was done in duplicate, as described in Materials and Methods.