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. Author manuscript; available in PMC: 2022 Apr 28.
Published in final edited form as: Biochemistry. 2022 Apr 5;61(8):689–702. doi: 10.1021/acs.biochem.1c00774

Figure 6.

Figure 6.

A618-versus-time traces demonstrating the effect of substrates on triggering the reaction of Fe(II)•BesC complex with O2 at 5°C. A reactant solution of BesC (0.20 mM), Fe(II) (0.40 mM, 2 molar equiv), and (A) L-Lys, (B) 6-OH-L-Nle, or (C) 4-thia-L-Lys at the concentrations indicated by the color-coded legends was mixed with an equal volume of O2-saturated buffer. The insets plot the amplitude of the fast phase (ΔA) as a function of the substrate concentration. The solid lines are fits of the quadratic (A and C) or hyperbolic (C) equation for binding to these data.