Validation of standard curve by analyzing known concentration of carnosine.

Carnosine concentration	Average AUC	Equation	Calculated concentration	Accuracy %	RSD %
10 nM	7383.00	y = 770.07x + 328.1	9.16 nM	91.61	5.30
500 nM	381 372.67	y = 770.07x + 328.1	494.82 nM	98.96	5.59
1.8 μM	1 366 184.00	y = 838484x − 91 003	1.74 μM	96.50	2.40
10 μM	8 109 971.00	y = 838484x − 91 003	9.78 μM	97.80	3.72