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. 2022 Apr 14;13:815828. doi: 10.3389/fimmu.2022.815828

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Copyright © 2022 Iyer, Hamers and Pillai

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Metal isotopes utilized in mass cytometry. (A) Periodic table summarizing the elements currently available for mass cytometry experiments. Grey, possible contaminating elements; green, elements used to indicate DNA content or cell size; purple, elements available for antibody conjugations; blue, elements available as probes and tracers; orange, elements not yet explored for mass cytometry but of potential future interest. Pink font; isotopes included in QE4 calibration beads. (B) Certain isotopes work well for the use in sample 1 barcoding. Shown is an example of palladium barcoding using 6 isotopes in unique combinations of 3. This strategy generates 20 separate barcodes, allowing 20 individual samples to be combined into 1 single tube.