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. 2022 Apr 14;13:815828. doi: 10.3389/fimmu.2022.815828

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Copyright © 2022 Iyer, Hamers and Pillai

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Step-by-step sample preparation. Representative sample preparation protocol. The order of individual steps may vary depending upon the experimental design (see text). Sample washes with cell staining buffer separate each step. The final 3 steps are specific to mass cytometry. PFA, paraformaldehyde.