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. 2022 Apr 14;13:826164. doi: 10.3389/fimmu.2022.826164

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Copyright © 2022 Feola, Russo, Martins, Lopes, Vandermeulen, Fluhler, De Giorgi, Fusciello, Pesonen, Ylösmäki, Antignani, Chiaro, Hamdan, Feodoroff, Grönholm and Cerullo

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Generation and characterization of AdOVA and AdTRP2 (A) Schematic representation of oncolytic adenovirus delta 24 (Ad5/3-D24) constructs with modifications in E1, E3, and fiber region. Both unarmed (Ad_unarmed) and armed (Ad_OVA and Ad_mTRP2) bear a deletion of 24bp in the E1A gene. Additionally, AdOVA (Ad_OVA) and AdTRP2 (Ad_mTRP2) contains an expression cassette under CMV promoter in E3 region. (B) Reverse-transcribed PCR reaction with specific primers for OVA and mTRP2 on cDNA derived from A549 and 4T1 infected with 5 and 100 MOI. (C, D) A549 cells were infected with 10MOI and the cell lysate was collected at 48h and OVA and TRP2 levels were checked by ELISA and the data are depicted as bar plots.